Name: Chapter 6 Quiz Indicate the answer choice that best completes the statemen

Question

Name: Chapter 6 Quiz Indicate the answer choice that best completes the statement or answers the question Class: Date 1. Which of the following is the most unique about an uncompetitive inhibi sor? a. It affects the KM of the enzyme b. It affects the Va of the enzyme c. It can be identified by a Lineweaver-Burk plot d. The inhibitor can bind to ES but not to fros E e. None of these is particularly unique to an uncompetitive inhibitor 2. According to the steady-state assumption a. the product concentration does not change significantly b. the substrate concentration is large and does not change significantly c. the concentration of enzyme-substrate complex remains constant with time d. the free enzyme concentration is always in great excess to the concentration of enzyme-substrate complex 3. What effect is seen on a Lineweaver-Burk graph when a mixed-type inhibitor is added? a. The y-intercept is changed, but not change the slope of the line. b. The slope of the line is changed, but not the y-intercept. c. Both the y-intercept and the slope of the line are changed. d. Neither the y-intercept not the slope of the line is changed. 4. The order of a reaction can be determined from the balanced equation for the reaction. a. True b. False 5. The KM expression is equal to a. (ki + k2)/k1 b. (k+k2)/k 6. If the y-intercept of a Lineweaver-Burk plot - 1.91 (sec/millimole) and the slope 75.3 sec, Kequals: 191 (se c millimole) and the slope-753 Ls.KM eluls 6. If they-intercept of a lineweaver-Burk plot a. 0.0254 millimolar (mM). b. 0.523 millimolar (mM). c. 5.23 millimolar (mM). d. 39.4 millimolar (mM). e. 75.3 millimolar (mM) Page 1

Explanation / Answer

Answer:

Chapter 6 Quiz

Indicate the anser choice that best completes the statement or answers the question.

1. d. The inhibitor can bibd to ES but not to free E

2. c. the concentration of enzyme-substrate complex remains constant with time

3. c. Both the y-intercept and the slope of the line are changed

4. b. False

5. b. (K-1 + K2) / K1

6. b. 0.523 millimolar (mM)

Calculation:

y-intercept = - 1/Km

(y-intercept is negative)

So, -1.91 = -1/Km (Cancelling "-")

Km = 1/1.91 = 0.523 millimolar (mM)

7. b. The activation energy

8. b. A non-competitive inhibitor

9. a. True

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