(1) On a balance weigh 0.5 grams of algae powder and -0.5 grams of anhydrous mag
ID: 981062 • Letter: #
Question
(1) On a balance weigh 0.5 grams of algae powder and -0.5 grams of anhydrous magnesium sulfate. (2) Transfer (1) to a large test tube and combine with 2.0 ml of acetone. Stopper the test tube and shake vigorously for approximately one minute. You need to make sure that the solvent and solid are well mixed. Allow the mixture to stand for few minutes. * Define extraction. * What do you think from this mixture is soluble in acetone? * What do you think from this mixture is insoluble in acetone? (3) Use a pipette to carefully transfer the solvent above the solid (should be green) into a small test tube. Cover the tube to avoid evaporation * If a significant amount of solvent is evaporated, is it okay to add few more drops of solvent at this point? Explain.Explanation / Answer
(2)
Extraction: Seperation of compounds from crude by using solvent is called as extraction.
If mixture is soluble in acetone then we can say that mixture is having organic compounds.
if mixture is not soluble in acetone then we can say that mixture is having some salts.
(3)
If solvent evoporates then we can add same solvent. So no chemical change in that mixture
Store a sharps waste container near where the sharps are generated.
(7)
Compond on the TLC may dissolve in mobile phase. Then it is very difficult to observe separation of compounds on TLC
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