2. (10 pts) Circle True or False for each of the following statements about spec
ID: 982056 • Letter: 2
Question
2. (10 pts) Circle True or False for each of the following statements about spectrophotometry: a. True / False: Percent transmission is proportional to concentration of a chromophore. b. True / False: The molar absorptivity of a chromophore is a constant for that chromophore, so if you know its value from one experiment you could use it in another experiment, given the same conditions. c. True / False: The molar absorptivity of a chromophore is characteristic of that chromophore, and thus has the same value regardless of wavelength being monitored. d. True / False: Beer’s Law predicts that for a fixed path length, absorbance will be proportional to concentration for any value of absorbance or concentration. e. True / False: A student needs to take an absorbance spectrum of a sample across all wavelengths from 250 to 700 nm. You should advise the student that it will be faster to use a singlebeam spectrophotometer instead of a photodiode array spectrophotometer.
Explanation / Answer
a) Percent transmission is proportional to concentration of a chromophore- TRUE, the given statement can be derived from the Lambert's Law
b) The molar absorptivity of a chromophore is a constant for that chromophore, so if you know its value from one experiment you could use it in another experiment, given the same conditions. - TRUE, this value is the constant which we can use for other equations
c) The molar absorptivity of a chromophore is characteristic of that chromophore, and thus has the same value regardless of wavelength being monitored. - FALSE
It depends on the wavelength of the light used
d) Beer’s Law predicts that for a fixed path length, absorbance will be proportional to concentration for any value of absorbance or concentration - TRUE
e) A student needs to take an absorbance spectrum of a sample across all wavelengths from 250 to 700nm - TRUE
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