Coenzyme A transferees catalyses the following reaction: Acetyl CoA + acetoaceta

Question

Coenzyme A transferees catalyses the following reaction: Acetyl CoA + acetoacetate acetate + acetoacetyl CoA From the given data below, determine the following: Is the mechanism sequential or ping-pong? What is the order of addition of substrates to the enzyme and release of products? Make sure your answer makes "mechanistic sense". The order shown above is not necessarily the correct order. NOTE: some are i.v. patterns in the absence of products while others are i.v. patterns in the presence of product.

Explanation / Answer

From graph it is clear the enzyme mechanism belongs to ping-pong and the oreder of reaction is second order

second rate = k[S1][S2] rate is proportional to the first power of each of two reactants

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