An experiment was conducted to determine the hairpin loop region in termination.

Question

An experiment was conducted to determine the hairpin loop region in termination. Two DNA templates were prepared and used in in vitro transcription assay. The samples were analyzed for release of the RNA polymerase.
Construct 1 - hairpin sequence removed; Construct 2 - two copies of hairpin sequence inserted; Construct 3 - normal DNA with only one hairpin sequence
Construct Level of truncated Nascent
RNA released (arbitrary units)
1 10
2 11
3 10

The data from this experiment suggest that
A. hairpin loops are not required for termination.
B. two hairpin loops rapidly destabilize the polymerase.
C. the data are inconclusive.
D. additional hairpin loops increase the stability of the RNA:DNA hybrid.
E. both hairpin loops are not required for termination and two hairpin loops rapidly destabilize the polymerase are possible explanations.

Explanation / Answer

Conclusions of experiments

Construct 1 - hairpin sequence removed; arbitrary units =10

It can be concluded there can be other mechanisms available for to terminate transcription

Construct 3 - normal DNA with only one hairpin sequence arbitrary units =10

Means hairpins terminates transcription effectvely along with othere mechanisms

Construct 2 - two copies of hairpin sequence inserted; arbitrary units = 11

More effective step for termination comparatively to first step and third step

so fourth options E best explains the experiments

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