First line - maker Second and fourth line are positive tube Third line and fifth
ID: 98366 • Letter: F
Question
First line - maker Second and fourth line are positive tube Third line and fifth are negative tube (Based on data table below) Restriction Enzyme Digestion and DNA gel Electrophoresis In-Class Questions Results & Analysis Compare the DNA size pattern between the lanes control undigested and the Hind Il and BamH I- digested rpARA, and determine the size of the obtained fragments. Answer the following questions: 1. How many bands do you observe in the lane of the undigested plasmid? 2. Do the band(s) in the previous lane represent circular or linear fragments? 3. If your answer to the previous question is more than one, what does each band represent? 4. How many bands do you observe in the lane of the digested plasmid? 5. Do the band(s) represent circular or linear fragments? 6. Use the known fragment sizes in the marker lane to estimate the sizes of the fragments you obtain af bhe tagnents you ob in the digested plasmid lane 7. The tomato gene is present on thebase pair (bp) fragment. 8. The amp, gene is pres ent on the base pair (bp) fragment.Explanation / Answer
1) The first column represents the control and the second column represents the electrophoretic pattern of undigested plasmids. There are three distinct bands in the second column.
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