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E. Write the protein sequence if there was an insertion as shown below. F. If a

ID: 98420 • Letter: E

Question

E. Write the protein sequence if there was an insertion as shown below.

F. If a cell containing the mutation described in “e” were plated on a petri dish and incubated

overnight, what would you predict would be the phenotype of the colony compared to a wild type

colony? Why?

G. Write the protein sequence if there was a deletion of the base pair shown below.

H. If a cell containing the mutation described in “g” were plated on a petri dish and incubated

overnight, what would you predict would be the phenotype of the colony compared to a wild type

colony? Why?

I. Write the protein sequence if there was an insertion as shown below.

J. If a cell containing the mutation described in “i” were plated on a petri dish and incubated

overnight, what would you predict would be the phenotype of the colony compared to a wild type

colony? Why?

E) Write the protein sequence if there was an insertion as shown below 3' -GCG-5' 5' -CGC-3" 3' AATGTGATCC GGCGCATACT TTAAATGGCA TCTTGCACTC GTAAATAATT TTGGCGATGT TGTCCACTCG 5 5' TTACACTAGG CCGCGTATGA AATTTACCGT AGAACGTGAG CATTTATTAA AACCGCTACA ACAGGTGAGC-3 -10 +1 10 21 31 41 51 F) If a cell containing the mutation described in “e" were plated on a petri dish and incubated overnight, what would you predict would be the phenotype of the colony compared to a wild type colony? Why? G) Write the protein sequence if there was a deletion of the base pair shown below. 3'-AATGTGATCC GGCGCATACT TTAAATGGCA TCTTGCACTC GTAAATAATT 5'-TTACACTAGG CCGCGTATGA AATTTACCGT AGAACGTGAG TTGGCGATGT TGTCCACTCG-5 CGCTACA ACAGGTGAGC-3 CATTTATTAA AAC 10 10 21 41 51

Explanation / Answer

I. Protein sequence is the sequence encoded by the genes to represent a good target for novel antibiotics covering different therapeutic profiles. The putative functions of some of these essential proteins, inferred by bioinformatic analysis are presented.