Question
First to discover bacteria in pond water a. John B. Gurdon b. Anton van Leeuwenhoek c. Martin Karplus d. Dmitri Ivanovsky and Theodor Svedberg e. Robert Hooke Which technique would be best for finding functional protein binding domains? a. Cell culture b. Somatic Cell Nuclear Transfer (SCNF) c. Protein mass spectrometry d. Protein chip microarrays e. Isoelectric focusing (IEF) Which is not a chemical element needed for human life? a. Oxygen b. Peptidoglycan c. Phosphorus d. Carbon e. Chromium A protein may lose or gain a proton when the molecule is placed in an aqueous solution. The exact probability that a molecule will be protonated or deprotonated depends on the pKa of individual amino acids. This is also known as the isoelectric point of the protein (pl). a. True b. False. What technique allows one to determine tertiary structure a. 2D PAGE b. Light microscopes c. Cell culture d. X-ray crystallography e. Mass spectrometry In 2D PAGE, the gel is setup with a pl that starts at 6.2 and increases to a pl of 6.8. If you placed the proteins at the starting end, which proteins move the farthest? a. the largest b. the smallest c. the proteins with a net zero charge at pH 6.2 d. the proteins with a pl of 6.5 e. the proteins with a pl of 6.6 In the "sizing" part of 2D PAGE, which proteins move the farthest during polyacrylamide gel electrophoresis? a. the largest the b. the smallest c. the most positive d. the proteins with a pl of 6.4 e. the proteins with a pl of 6.6
Explanation / Answer
48)b Anton van Leeuwenhoek
50b Peptidoglycan is not a chemical element so it is the answer.
52) d X ray crystallography
