It is known gene H in chickens is responsible for fatty acid metabolism. You won

Question

It is known gene H in chickens is responsible for fatty acid metabolism. You wondered if bovines possess a homologous gene even though we know that the chicken is a non-mammal and non-rumen animal. You are asked to design an experiment to prove or disprove the idea that the bovine genome contains a gene H homolog. Purpose a set of experiments using what we have learned in class. Make sure to include the platform you are going to use, why you chose that particular system, and a brief description of the experimental design (times, control, sample collections, etc.), analysis, and data interpretation.

Explanation / Answer

One should do a DNA microarray analysis since its just a comparative study to find the presence or absence of a gene. Affymetrix or agilent have generated bovine while genome microarray chips which cud be used for this purpose.

Isolate gDNA of chicken and label gene H with Cy3 and GAPDH gene with Cy5 an vice versa (for dual channel) during amplification step. The former is your test sample while GAPDH will serve as your control.

NOTE: You can obtain chicken blood samples from slaughter houses and isolate gDNA(genomic DNA from them).

Mixed labelled samples with hybridization solution

Add the mixture to microarray pinholes and is allowed to be mixed by a mixer or a rotor.

The microarray is then dried, scanned and recorded

Raw data is normalised using appropriate/ platform-compatible method. Eg. RMA for affymetrix. This is done to get the total intensities at comparable levels.

Hence if significant fluorescent signal is obtained for gene H it would that a similar gene (homolog) is also present in bovine otherwise not.

Related Questions

Navigate

• Browse (All)
• Browse I
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.