Toluene has a polarity in between the two other compounds. In what order would t

Question

Toluene has a polarity in between the two other compounds. In what order would the compounds elute in normal-phase HPLC? For a given mobile phase composition in normal phase HPLC, the retention time of ethylene glycol is 5.6 minutes. Will the retention time increase or decrease by increasing the polarity of the mobile phase? In what order would the compounds elute in reversed phase HPLC? For a given mobile phase composition in reversed phase HPLC, the retention time of hexane is 5.6 minutes. Will the retention time increase or decrease by increasing the polarity of the mobile phase?

Explanation / Answer

a

in the normal phase HPLC

compounds will come according to the polarity means

less polar compound will come first more polar compound will come last

tolune will come in wetween these two

hexane will come first

ethylene gly col will come last

b

if you increase the polarity spotis will come even much quickly so retention time will decrease

c

reverse phase HPLC means exactly reverse tot he normal phase column

so here first more polar compound will come last least polar compound will come

ethylene glycol is first

toluene second

hexane is last

d

if you increase the polarity here also retentime will decrease

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