43 . (6 points) You have isolated 2 new recessive drosophila mutants, double-win

Question

43. (6 points) You have isolated 2 new recessive drosophila mutants, double-wing and long abdomen, and are doing linkage analysis of the mutants to each other. Starting from purebred for each mutant.

a.Diagram how you would test for linkage.

b.Make a table of expected results assuming independent assortment if you had 400 progeny.

c.Add a column of observed results if there was in fact linkage with a recombination frequency of 20%

44. Using the following piece of DNA:

a.Draw the complimentary strand

b.Assuming RNA polymerase reads the bottom strand, Label the coding and template strands.

c.draw the transcribed mRNA.

d.Write the amino acid sequence.

e.What are the anticodons of the tRNAs?

Please give a good explanation to your answer, will give a thumbs up if correct, thank you. (visual learner)

SECOND BASE UCU UAU UAC UAA Stop UGU- UGC- Cys UGA Stop A Phe UUC UUA UUG UCC -Ser Leu UCG UAG Stop UGG Trp CCA Pro, CAC1--His C CCG CGU CGC CGA CGG- AGU CCU CAU CUC Leu Pro CAC His c Arg WCUA CAG Ser ACC AAALYSAGG AUClle ACC ACA AUG Stant ACG GCU AAC AUA Met or AAG Lys AGA AGG GAU- GUC GGC Gly GUAVal GCC | GAA GGA Glu GUG GAG

Explanation / Answer

The DNA strand is,

5'- CAG ATG GCC TGT CAC TGA G -3'

a) The complimentary strand is,

3'- GTC GAC CGG ACA GTG ACT C -5'

b) Coding strand is 5' - CAG ATG GCC TGT CAC TGA G-3'

Template strand is 3'- GTC GAC CGG ACA GTG ACT C-5'

c) Transcribed mRNA is GUC GAC CGG ACA GUG ACU C

d) amino acid sequence is Val-Asp-Arg-Thr-Val-Thr

e) anticodons of tRNA: CAG CUG GCC UGU CAC UGA G

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