Write your response to the following questions directly on the test packet. Usin

Question

Write your response to the following questions directly on the test packet. Using the following piece of DNA (a) Draw the complimentary (as the bottom strand) (b) Assuming RNA polymerase reads the top strand, Label the template & coding strands. (c) draw the transcribed mRNA. (d) Write the amino acid sequence (e) Give the anti-codon of the 1^st two tRNAS (f) From the previous question, replacing the C at the 7th position (underlined) with a G would result in what kind of mutation? 5'-TCATGTC_TT CGTTATCCTAGTCATC-3'

Explanation / Answer

a)5'-AGTACAGAAGCAATAGGATCAGTAG-3'

B)coding strand - 5'- AGTACAGAAGCAATAGGATCAGTAG-3'

template strand- 3'- TCATGTCTTCGTTATCCTAGTCATC-5'

C)transcribed mRNA strand -3'-AGUACAGAAGCAAUAGGAUCAGUAG-5'

D)amino acid sequence =ser-thr-glu-ala-ile-arg-ile-ser

e)TGA,TGT

F)missence mutation-where a base is substituted with another leading to the synthesis of altered amino acid.

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