As its name implies. fluorescein fluoresces describe the electronic transition o
ID: 995202 • Letter: A
Question
As its name implies. fluorescein fluoresces describe the electronic transition of ground or excited singlet or triplet state that occurs during fluorescence. State the relationship of the rate constants for radiation less transition versus fluorescence in the transition step described in 1). fluorescein appears to have two colors: a yellowish-green and orange One is its absorption color and the other is its fluorescence color The two lambda max values are at 570 nm and 605 nm. Which is the absorbance wavelength and which is the fluorescence wavelength? (Flint: which must be the longer wavelength?)Explanation / Answer
1)flurocence caused by a radiative transition between sates of same multiplicity . i.e ; singlet to singlet, triplet to triplet. s1 to s0 is the most common.
2) raditionless transitions rate constat ir iversly proportional to the rate constant of fluroescence .
3) absorbance wave length should be lower than the fluorescnce wave length beacuse when absorb energy molecules goes to higher vibrational levels of the excited satete. then they have to come down to the lowest vib energy state of excited satate before its undergo fluorecnce. due to this vibrational relaxation some enery will lost as thermal energy,, then remaining energy of excited molecule will release as fluorocence and comes dwn to the ground state. thus high energy (low wave length) for absorbance , low energy(high wave length) for the fluorocence.
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