So I want to create a 100 mL solution of 1 micro molar fluorophore and 1 micro m

Question

So I want to create a 100 mL solution of 1 micro molar fluorophore and 1 micro molar potassium cyanide in PBS for an experiment. I am wondering if making a diluted solution from two stock solutions is the same as making a dilution from a single stock solution. for example if I have a 50 micro molar stock solution of fluorophore, and a 10 micro molar stock solution of KCN and I want to make a 100mL, 1 micro molar fluorophore and 1 micro molar KCN solution with PBS, do I just solve m1*v1 = m2*v2 for v1 for both the KCN and fluorophore and then subtract the sum of the v1's of both fluorophore and KCN from 100ml and add that much PBS to the two v1's of stock solutions?

Sorry if this is wordy let me know if I need to clarify..

Explanation / Answer

The method you have described is the correct method. But in some instances, when two concentrated solutions are mixed, they may react with each other. Hence, it is better to mix two diluted solutions.

(1) You dilute v1 ml of stock solution of fluorophore with some PBS

(2) You dilute v1 ml of stock solution of KCN with some PBS.

Shake these two solutions thoroughly and then mix these two solutions thoroughly.

Then dilute the mixture with PBS to a total volume of 100 mL.

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