1) Why does the spectrometer need to be warmed up for 15 min prior to using? 2)

Question

1) Why does the spectrometer need to be warmed up for 15 min prior to using?

2) Why do cuvettes need to be conditioned? That is, rinsed three times with the solution to be analyzed.

3) Why do we calibrate at each wavelength in % transmission mode?

4) A serial dilution is performed where a stock solution is diluted by half, and the new solution is diluted by half. What is the relative concentration of the new/final solution made?

The following data was collected. What is the equation of the line, in the form of y=mx+b (Show your work).

Explanation / Answer

1) a) instrument drift

2) b)To avoid cross-contamination

3) d) so the instrument knows what no light, and 'all' light is like

4) d)0.25 ; intially 1X concentration after half dilution 0.5 and after diluting it with half we get 0.25 X concentration

5) f) y = 0.9976x + 0.0062

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