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If a cell were unable to produce histone proteins, which of the following would

ID: 99986 • Letter: I

Question

If a cell were unable to produce histone proteins, which of the following would be a likely effect? A) Spindle fibers would not form during prophase. B) DNA polymerase I would not function properly. C) Expression of other genes would compensate for the lack of histones. D) The cell's DNA couldn't be packed into its nucleus. Which of the following statements accurately describes why Tag polymerase is used in PCR? A) It has regions that are complementary to the primers. B) It is heat stable, and it binds more readily than other polymerases to the primers. C) Only minute amounts are needed for each cycle of PCR. D) It binds more readily than other polymerases to the primers. E) It is heat stable and can withstand the heating step of PCR. In the late 1950s, Meselson and Stahl grew bacteria in a medium containing "heavy" nitrogen (15_N) and then transferred them to a median 14_N.Which of the results in Figure 13.1 would be expected after one round of DNA replication in the presence of 14_N? A) A B) B C) C D) D E) E Which enzyme was used to produce the molecule in Figure 13.3? A) DNA polymerase B) RNA polymerase C) a restriction enzyme D) ligase

Explanation / Answer

28- answer= D if cell is unable to produce histone protein the cells DNA could not be packed. In nucleus DNA packed with histone protein to form nucleosome. And then take part in coilling and supercoiling of DNA fibre.

29. Answer-E taq polymerase is heat stable and with stand with the PCR during amplification of gene. This enzyme is obtained from thermophilic bacteria and this enzyme is stable up to 78 degree centigrade so it is used in PCR amplification which occurs at very high temperature

30- answer D. initially bacteria have both the strand of DNA with N 15. During replication each stand act as template on which the new strand of N14 is polymerised. Now both the new strends have hundred percent hybrid character.

31. Answer C. Restriction endonuclease enzyme is responsible for the cutting of DNA from a specific position. This position is also known as palindromic site.

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