What are the differences between prokaryotic and eukaryotic gene expression in t
ID: 100173 • Letter: W
Question
What are the differences between prokaryotic and eukaryotic gene expression in terms of where the mRNA is transcribed, the initiation of mRNA transcription, and any post transcription modifications of the mRNA molecule. For the following DNA template strand, please give the corrected transcribed mRNA strand and the polypeptide that it codes for. Beware of start and stop codons. 3'- TCCTGATACGACTGAGGACTCTTCTTCAGA ATTGGA-5' Enzyme function is critically important for the proper replication of DNA. Predict the consequence of a loss of function for each of the following enzymes: a) DNA gyrase b) DNA polymerase III c) DNA ligase d) DNA polymerase I You are on an episode of CSI Grayslake. A crime has been committed and you are called in to put one of 3 suspects at the crime scene. You have a few hair stands with cells attached but not enough DNA to run an RFLP fingerprint assay. Map out a plan on how you would proceed to produce enough DNA for a specific gene that you will digest to get a DNA fingerprint. Include any protocols, enzymes, materials, etc that you would use. Describe in detail, translation of an mRNA by a ribosome. Include the function of rRNA and tRNA and associated enzymes or complexes.Explanation / Answer
Question 1 Answer:
Phenomenon
Prokaryotic Trnascription
Eukaryotic transcription
Occurs in
Cytoplasm
Nucleus
No. of. Polymeases
Single polymerase
Three polymeases
No. of subunits in RNA polymerase
5
10-15
RNA released in
Cytoplasm
Nucleus
Cystronic unit
Polycystronic
Monocystronic
Initiation
Does not need initiation factor
Initiation factors required
Post transcriptional modifications
Absent
Three modifications presnt
Capping,
Poly Adenylation and
splicing
Question 2 Answer:
Gene: 3'TCCTGATACGACTGAGGACTCTTCTTCAGAATTGGA5'
mRNA: 5'AGGAGUAUGCUGACUCCUGAGAAGAAGUCUUCCCCU3'
In RNA, the complementary formula is A=U and G=C.
In this gene sequence, the starting codon AUG and found two non sence codons.
ACCORDING TO CHEGG GUIDELINES WE HAVE TO ANSWER ONE QUESTION AT A TIME. SO POST THE REST AS SEPERATE QUESTIONS THEN I CAN HELP YOU.
Phenomenon
Prokaryotic Trnascription
Eukaryotic transcription
Occurs in
Cytoplasm
Nucleus
No. of. Polymeases
Single polymerase
Three polymeases
No. of subunits in RNA polymerase
5
10-15
RNA released in
Cytoplasm
Nucleus
Cystronic unit
Polycystronic
Monocystronic
Initiation
Does not need initiation factor
Initiation factors required
Post transcriptional modifications
Absent
Three modifications presnt
Capping,
Poly Adenylation and
splicing
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