While working in the lab, you find 6 deletion mutants in the bread mold Neurospo

Question

While working in the lab, you find 6 deletion mutants in the bread mold Neurospora crassa that make them auxotrophic for lysine i.e. they require lysine supplementation). The spores for each mutant are mated with each other to form a diploid cell that is then plated on minimal media (MM) without lysine. You examine the results of each mating to see if there is growth on MM. "-" in the table below indicates no growth on MM while "+" indicates growth on MM. a. Fill in the missing cells in this table (Five points). The "//" means you do not need to fill that box in. You find four new mutants that are in the same complementation group and result in mutants that require lysine supplementation to grow on MM. Upon analysis you also discover that these mutations are deletions of key nucleotides. The table of a complementation analysis involving these 4 deletion mutants is shown below. Also shown is a map of where the regions containing these deletions are located along the length of the gene (black line) Possible region of Deletion: Armed with this information, you discover 6 new point mutations (a through f) in the same gene mapped, above. You cross them with the deletions and plate the diploids onto MM (i.e. no lysine). Thu results arc shown in the table above. Remember that "-" means no growth was observed. Take the gene given above and modify it to shown whore these point mutations should be located on this gene.

Explanation / Answer

1 2 3 4 5 6 1 + + - 2 + + 3 + + 4 + 5 6

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