You have discovered a strain of E. coli that grows very slowly-the generation ti

Question

You have discovered a strain of E. coli that grows very slowly-the generation time is nearly 12 hours compared to the normal 20-30 minutes. Upon further investigation, you find a mutation in the DNA polymerase III gene. What subunit of the holoenzyme does this mutation affect the most? You extract DNA from an E. coli cell and observe it is hemi-methylated. Which strand of DNA is older? The DNA strand replicated in vitro is: GGGCCATTCGAACGTCCGAAAATGCCCCTGAATGAAAATTTTGGCCC. The primer used for replication is CCCGGTAAGCTT. Where is the 5' end for the template and primer, respectively?

Explanation / Answer

3) You have discovered a strain of E. coli that grows very slowly - the generation time is nearly 12 hours compared to the normal 20-30 minutes. Upon further investigation, you find a mutation in the DNA polymerase III gene. (beta) subunit of the holoenzyme does this mutation affect the most.

4) You extract DNA from an E. coli cell and observe it is hemi-methylated. The methylated strand of DNA is older.

5) The DNA strand replicated in vitro is 5'-GGGCCATTCGAACGTCCGAAAATGCCCCTGAATGAAAATTTTGGCCC-3'.the primer used for replication is 3'-CCCGGTAAGCTT-5'. An Okazaki fragment is a small fragment of DNA with no RNA primer at the 5' terminus formed on the lagging strand during DNA replication.

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