Can the lytic P1 phage infect the lysogen produced by integration of the phage d
ID: 101587 • Letter: C
Question
Can the lytic P1 phage infect the lysogen produced by integration of the phage described in question a? Explain your answer.A temperate bacteriophage has the gene order x1 x2 x3att x4 x5.
a.) What is the gene order of the prophage?
b.) Can the lytic P1 phage infect the lysogen produced by integration of the phage described in question a? Explain your answer. (Hint:The phage in part a is Lambda.)
c.) Describe the sequence of events that occur when the virulent phage T4 infects susceptible host cells.
A new series of rII mutations in the virulent phage T4 has been isolated, and the results of phage recombination experiments between all twelve of the new mutations are listed below:
d.) Draw a linear genetic map showing the location of all point mutations and the frequency of recombination between adjacent mutations. Describe the location and extent of all deletions.
1 23 4 5 6 78 9 10 11 12 1 0 0.2 3.5 0.5 2.8 1.5 2.01.9 | 0.7 1.0 0.5 3.0 0 3.7 0.7 3.0 1.72.2 2.1 0.91.2 0.3 3.2 0 3.00 2.01.51.6 1.42.5 4.0 0.5 0 2.3 1.0 1.5 1.4 0.2 0.5 1.0 2.5 2 0 1.3 0.8 0.9 0.71.8 3.3 0 0 0.5 0.4 0 0.5 2.0 1.5 0 0.1 0 1.0 2.5 1.0 0 00.92.4 1.1 0 0 1.2 0.9 0 1.5 2.0 0 3.5 10 12Explanation / Answer
A. ANS: According to the given question the temperate bacteriophage has the gene order x1 x2 x3att x4 x5. For obtaining bacteriophage prophage we should first recombine the bacteria at the 'att' site. The bacteria 'att' site was lacated between the 'gal' and 'bio' genes. After the recombination the prophage can contains two cos sites which are comprised of 'att' sites on both the ends. So the gene order of the prophage is gal-att-x4-x5-x1-x2-x3-att-bio.
B. ANS: The lytic P1 phage does not infect the lysogen. It is because the the lysogenic bacteria already contain a prophage. So the existing prophage can significantly inhibit the other bacteriophage infection.
C. ANS: Virulent T4 phage is a virulent phage which undergoes only lytic cycle, so the sequence of events is listed below:
1. Attachment: Phage T4 gets attached to the surface of the host cell.
2. Penetration: T4 phage then injects its genetic material inside the host cell.
3. Biosynthesis: Viral mRNA hijacks the host replication machinery and transcribes the viral proteins.
4. Assembly/maturation of virion: Synthesized proteins are assembled to form new mature virions.
5. Cell lysis: When the enough number of viral copies is synthesized, T4 directs the lysin enzyme production for breaking bacterial cell wall and release of new T4 virulent phages.
Please consider this: Answering to many questions is against to chegg rule.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.