When DNA extraction and isolation is completed, it is typical to ascertain how m

Question

When DNA extraction and isolation is completed, it is typical to ascertain how much DNA was successfully extracted. To do this, a sample of the extract is diluted and placed into a UV-Visible spectrometer. A spectrum is collected and the graph evaluated at a specific wavelength. What is the wavelength? nm Why do we look at this specific wavelength? The absorption properties of DNA can be used for detection, quantification and assessment of purity. The wavelength of maximum absorption for both DNA and RNA is 280 nm (lambda_max = 280 nm) with a characteristic value for each base. The wavelength of maximum absorption for both DNA and RNA is 260 nm (lambda_max = 260 nm) with a characteristic value for each base. At a concentration of 1 mu g/mL and a 1 cm path length, dsDNA has A_260 = 50.

Explanation / Answer

1) Measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm is used as a measure of DNA purity

2) the absorption properties of DNA can be used for detection, qualification and assessment of purity.

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.