1) Why is it important to seek out natural antimicrobial products? _____________
ID: 102086 • Letter: 1
Question
1) Why is it important to seek out natural antimicrobial products?
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6) What is a “satellite colony” that you observed during the zone of inhibition lab and what information does it give you? ( 2points)
(This is the answer I wrote, but im not too confident about it)
-Satellite colony is a number of small bacterial colonies that surround a larger antibiotic resistant colony. It gives us the information on how effective the antimicrobial agent by measuring the distance of the zone of inhibition.
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8) You have obtained your results from Lab. You notice that the zone of inhibition for your agar plates vary from the results of your classmate next to you. Please explain why this was observed. (1 point)
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11) Plate 0 minutes has 3 colonies, Plate 30 minutes has 0 colonies, Plate 90 minutes has 6 colonies.
A) explain why there are no colonies at 30 minutes.
B) explain why there are more colonies at 90 minutes.
Explanation / Answer
1. now a days mostly natural antimicrobial products are only supplied in the markets and used as well.
as due to the increased incidence of the drug resistance to many pathogens the attention of the pharmaceuticals and the scientific communities have been toward the studies relating to the naturally produced products.
the misuse and mis handling of the antibiotics led to the rise in micro organisms which has also been one of the criteria for the use of naturals.
compounds derived from the natural sources have potential to be used against a broad range of pathogens, some plant derived products are most active against a particular type of bacteria or fungi.
many natural products from different sources have been supplied like from plants, animals, bacteria, algae, fungi etc..
6. the answer given above is correct about satellite colony
they are the small numerous bacterial colonies that surround the antibiotic resistant colony on the nutrient agar plate.
they appear because beta lactamase secreted by the transformed vector cells degrade the antibiotics around the transformed cells leading to the growth of non transformed colonies.
these colonies also grow when the pH of the medium of below 7.
common example of these colonies are generally seen in e.coli and ampicillin containing agar plates.
these satellites are not a problem as they will not grow when transferred to a medium containing fresh antibiotic.
8. zone of inhibition vary from other
if any antibiotic stops the bacteria from growing or kills the bacteria then no bacteria is seen on the nutrient agar plates, it is called zone of inhibition.
the size of this zone usually depends on how effective the antibiotic is at stopping the growth of bacterium, and also depends on the concentration of the antibiotic.
so, the zone differs from other as may be the concentration of the antibiotic apllied differs or different antibiotics were used on different plates so leads to difference in killing the bacterium.
11.A) after 30 min no colonies were found as stronger the antibiotic used on the agar plates then the growth of the bacterium stops or else if the concentration of the antibiotic used is more then it creates larger zone of inhibition and less amount of the solution is enough to kill the bacteria, so basing on any of the 2 reasons the plate did not show colonies.
11B) plate at 90min showed more colonies, as may be the concentration of the antibiotic used on the plate isn't sufficient to kill all the bacteria present or may be the type of antibiotic solution is not of such strong to inhibit the type of microbial growth so the colonies were observed, which can be destroyed on using some other type of strong antibiotic.
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