Comparison of embryonic human liver miRNA profiling studies with the chick profi

Question

Comparison of embryonic human liver miRNA profiling studies with the chick profiles found that several miRNAs appear to be expressed differently between the two species. For example, both miR-100 and miR-130b seem to be expressed higher in the embryonic avian liver than in the embryonic human liver. How would you design an experiment to determine which pathways these miRNAs regulate in the chick liver? Be sure to include an explanation of how each component of your experiment will aid you in identifying these pathways. You may use the luciferase assay, but you also need to include additional experiments to provide a more complete analysis of these miRNAs.

Explanation / Answer

Offcourse the experiment has to be based on the comparison of the chick liver cells with and without the miRNA expression.

This can be done by knocking out the gene responsible for miRNA in cells or curbing the transcription of the miRNA. Comparing the pathways in miRNA positive and miRNA negative cells you can trace out the pathways that are regulated by miRNA.

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