Comparison of embryonic human liver miRNA profiling studies with the chick profi

Question

Comparison of embryonic human liver miRNA profiling studies with the chick profiles found that several miRNAs appear to be expressed differently between the two species. For example, both miR-100 and miR-130b seem to be expressed higher in the embryonic avian liver than in the embryonic human liver. How would you design an experiment to determine which pathways these miRNAs regulate in the chick liver? Be sure to include an explanation of how each component of your experiment will aid you in identifying these pathways. You may use the luciferase assay, but you also need to include additional experiments to provide a more complete analysis of these miRNAs.

Explanation / Answer

Collect chick liver samples and prepare them for isolating RNA from the liver.

Use RNA/protein spin columns to extract RNA.

Now use miRNA extraction kit and to isolate miRNA.

Now to find out the miRNA profiling, we do microarray procedure, in which each well of mircro array has different kind of miRNA probe and then we add extracted miRNA to each well.

After that identify the probe signified the miRNA through Fluorescence Spectroscopy and then observe the results and interpret that.

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