100 60 min 50 1324117391812 You have isolated a novel alien bacterium. You decid

Question

100 60 min 50 1324117391812 You have isolated a novel alien bacterium. You decide to determine the directionality of protein synthesis using the labeling method developed by Dr. Dintzis. You radiolabel nascent (newly synthesized) polypeptides during active translation with ['HLeucine and then digest the resultant proteins with an enzyme, which cuts at specific locations in the protein. You plot the relative radiolabel incorporation (Y-axis) versus polypeptide position (relative order within protein sequence) (X axis) and achieve the results shown here. You can conclude from this experiment that the alien bacterium protein synthesis occurs:

Explanation / Answer

The directionality of protein synthesis always occurs from the amino terminus of the polypeptide the carboxylate terminus of the polypeptide. The mRNA will be read in a 5' to 3' direction. Hence according to this the answer will be

Option D) Rrom N terminus to C terminus

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