The following experiment investigates the activity of an enzyme peroxidase and d

Question

The following experiment investigates the activity of an enzyme peroxidase and determines the effect of varying the enzyme concentration. Peroxidase catalyzes the breakdown of hydrogen peroxide with the production of water and oxygen. Certain dyes will go from a colorless to a colored form upon oxidation, a property that allows detection of peroxidase activity. In the presence of hydrogen peroxide and peroxidase, orthophenylenediamine (OPD) will be changed from a colorless dye to a yellow compound as it is oxidized Using a spectrophotometer, you quantitated the enzyme reaction by measuring the absorbency from the oxidized OPD at 410nm. The more colored OPD produced in a given time period, the faster the enzymatic reaction. The following are the contents of experimental test tubes(Table 1) and the absorbance readings: A. Write a hypothesis for this experiment B. What is the substrate of the enzyme peroxidase in this experiment? C. Which test tube contains the experiment designed as "no enzyme control" (see Table 1)?

Explanation / Answer

1)Hypothesis -
The OPD is converted to the yellow colour by oxidation in the presence of peroxidase by breakdown of hydrogen peroxide.
2) Hydrogen peroxide is the substrate for the enzyme peroxidase.
3) Tube number 1 contains the experiment designed as no enzyme control.

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