The following experiment investigates the activity of an enzyme peroxidase and d

Question

The following experiment investigates the activity of an enzyme peroxidase and determines the effect of varying the enzyme concentration. Peroxidase catalyzes the breakdown of hydrogen peroxide with the production of water and oxygen. Certain dyes will go from a and colorless to a colored form upon oxidation, a property that allows detection of peroxidase activity. In the presence of hydrogen peroxide and peroxidase, orthophenylenediamine (OPD) will be changed from a colorless dye to a yellow compound as it is oxidized. Using a spectrophotometer, you quantitated the enzyme reaction by measuring the absorbency from the oxidized OPD at 410nm. The more colored OPD produced in a given time period, the faster the enzymatic reaction. The following are the contents of experimental test tubes and the absorbance readings: A. Write a hypothesis for this experiment B. What is the substrate of the enzyme peroxidase in this experiment? C. Which test tube contains the experiment designed as "no enzyme control" (see Table 1)?

Explanation / Answer

1. HYPOTHESIS - for this experiment will be the Activity of OPD in some microorganism

2. The substrte in this experiment for enzyme peroxidase is OPD ie the orthophenylenediamine.

3.The second tube shows the noenzyme control because this is the hydrogen peroxide which control the OPD to change , and in this experiment it is zero that is no control to it.

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.