Cathade Using immunoprecipitation, you can isolate a protein (protein X) you thi

Question

Cathade Using immunoprecipitation, you can isolate a protein (protein X) you think is involved in chronic myelogenous leukemia (CML), which is caused, in part, by a hyperactive tyrosine protein kinase called ABL. You think one of the targets of ABL is the protein X that you can purify. In comparing tissues with and without the disease, you subject the samples to isoelectic focusing. Understanding how IEF works, how would you expect the samples with CML to migrate compared to the wild type, non-diseased sample protein? Use the figure to help you consider your response. p: 55 Choose one: A. The phosphorylated protein will have a lower isoelectric point and thus migrate further toward the anode. B. The phosphorylated protein will be more attracted to the negative charge on the anode. C. The non-phosphorylated proteins from the non-diseased samples will move further to the anode without the bulky phosphate group attached to a tyrosine. D. Non-diseased proteins will migrate with a lower pl than those from diseased tissues and thus the samples from the non-diseased samples will migrate more to the anode than diseased protein.

Explanation / Answer

1.A because phosphorylated molecules will have high negative charg and hence less pI value.

2 A,B.SDS is a detergent help in denaturing the proteins and provides suffiecient charge to that proteins to make them neutral.

3.D monoclonal antibodies which can bind to unique domain will be preferred most.

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