Paper Chromatography of Food Dyes Pre-lab Assignment 1. Separations by partition
ID: 1052156 • Letter: P
Question
Paper Chromatography of Food Dyes Pre-lab Assignment 1. Separations by partition chromatography depend on partitioning of a mixture's components between two solvents - a stationary phase on a solid support and a moving phase. What is the solvent of the stationary phase in paper chromatography? The solvent of the stationary phase is adsorbed onto the surface ofa solid support. What is the solid support in paper chromatography? 2· The mobile phase in partition chromatography can be a single solvent or a mixture of solvents depending on the particular separation desired. In the paper chromatography of food dyes (today's exercise), what is the composition of the mobile phase? 3. 4. Is the mobile phase more polar or less polar than the stationary phase? Explain your answer in terms of nonbonding attractive forces. There are seven FDA certified food colors, but McCormick Food Colors & Egg Dye contains only FD&C; Yellow #5, FD&C; Red #40, FD&C; Blue # 1, and FD&C; Red #3. (The food colors also contain water, propylene glycol, and 0.1% propylparaben (as solvent, humectant, and preservative, respectively.) 5. What is the common name of each of the following colors? FD&C; Yellow 5 FD&C; Red 40 FD&C; Blue1 FD&C; Red3
Explanation / Answer
1. the solvent of stationary phase is water.
2. The solid support is the paper used in the chromatography.
3. The solution used in this experiment is generally 0.1% naCl solution.
4. The attraction between the sample and the chromatography paper determines how much time the sample spends bonded to the paper and how much time it remains in solution. These factors determine the distance/time a sample will move.
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