First, you must read or re-read the section in the text on Spectrophotometric An

Question

First, you must read or re-read the section in the text on Spectrophotometric Analysis. The Beer-Lambert law is: A = E-t-c Where A is the, and has units of. and E is the, and has units of. and t is the, and has units of. and c is the, and has units of. A student is obtaining data to prepare a calibration curve for the absorption of CoCl_2(aq) as a function of concentration. This table contains the first two data points. After all the data was collected, the following linear calibration curve was obtained: y = 4.8048.x + 0.0021 If the absorbance is 0.446 (A) of a solution affected if a solution is diluted to twice the initial volume? You used spectrophotometry to measure the blood salicylate level in three patients. What should your dosage report indicate? Patient 1 shows a blood salicylate concentration of 625 mg/L? Patient 2 shows a blood salicylate concentration of 25 mg/L? Patient 3 shows a blood salicylate concentration of 285 mg/L? Match the following three questions with the correct answer.

Explanation / Answer

Q1.

a) A is absorbance, unitless

b) e is wavelength-dependent molar absorptivity coefficient with units of M-1 cm-1

c) l is the path length, typically in centimeter, cm

d) c is concentration, either mol/L or mg/L (must be same units as e)

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