A heptapeptide contained the following amino acids: Asp, Leu, Lys, Met. Phc, Tyr

Question

A heptapeptide contained the following amino acids: Asp, Leu, Lys, Met. Phc, Tyr. The following facts were observed during sequencing: Edman degradation resulted in a PTH-derivative, containing Phe side chain. Trypsin treatment had no apparent effect. Chymotrypsin treatment yielded several products, including a dipeptide and a tetrapeptide. The amino acid composition of tetrapeptide was Leu. Lys. and Met. Cyanogen bromide (CNBr) treatment yielded a dipeptide. a tetrapeptide. and free Lys. Determine the amino acid sequence of this peptide based on the above information. Elaborate your reasoningto receive FULL credits. Write the sequence in: 1) 3-letter and, 2) 1-letter abbreviations.

Explanation / Answer

                                                 N-Phe Asp Tyr Met Leu Met Lys-C

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