A chemical reaction catalyzed by the enzyme acid phosphatase is shown in the int

Question

A chemical reaction catalyzed by the enzyme acid phosphatase is shown in the introduction. How would you classify this enzyme in terms of the enzyme classification scheme described in the text book, i.e., oxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase? List at least four factors that can influence enzyme activity and indicate what is meant by the term "enzyme activity" in this context (see the text book). Considering the biochemical (protein) nature of enzymes, what are some factors or chemical and physical agents that can destroy or denature enzymes? Consider the earlier experiment regarding properties of proteins. You should list at least three.

Explanation / Answer

1. Concluding from the fact that enzyme acid phosphatase is used, it can be classified as HYDROLASE, because it catalyses the hydrolysis step of the reaction, i.e.

p-Nirtophenolphosphate -------> p-Nitrophenol

2. Enzyme Activity is basically the ability of an enzyme to convert a substrate into its product. Enzymatic activity can be measured by either measuring the rate of disappearance of the sustrate or the rate of generation of product.

Enzyme Activity = Total Moles of substrate converted per unit time = rate of conversion of substrate x volume of reactor/reaction vessel

The factors which affect this rate, and thus the enzyme activity are:

3. Considering the enzyme as "Protein-Natured", we can list the following possible reasons for denaturation of enzymes :

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