HPLC stands for (a) High Pressure Liquid Chromatography (b) High Performance Liq
ID: 1072588 • Letter: H
Question
HPLC stands for (a) High Pressure Liquid Chromatography (b) High Performance Liquid Chromatography (c) Highly Placed Liquid Chromatography A source of error in liquid chromatography is (a) allowing solvent to drain below the top of the stationary phase. (b) using a slow flow rate. (c) filling the column with a slurry of stationary solid phase rather than with the solid phase alone Normal-phase liquid chromatography refers to (a) the use of a nonpolar solvent as the eluent in HPLC. (b) the use of a polar material as the stationary phase in a chromatographic column. (c) the use of a nonpolar material as the stationary phase in a chromatographic column.Explanation / Answer
1) option B is correct
High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent at high pressure through a column with chromatographic packing material.
2) A is correct
Some special substances from the samples can be adsorbed strongly in and on the stationary phase. This changes the overall chromatography behavior of the HPLC column stepwise from run to run
3) option A is correct
Hydrophobic molecules can be eluted from the column by decreasing the polarity of the mobile phase using an organic (non-polar) solvent, which reduces hydrophobic interactions.
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