HPLC M ethod Development Sample Components - H H-o o-H Question: Please indicate
ID: 711828 • Letter: H
Question
HPLC M ethod Development Sample Components - H H-o o-H Question: Please indicate the conditions you would recommend as a starting place for designing the above four molecules. Whe appropriate a separation cf re options are provided please select the one which you feel is most Conditions: Column: C18 Silica C8 Silica Column Size: 4.6 x 15cm2.1x 10cm Detector: ELSD Initial Gradient Condition: Final Gradient Condition: Sample dissolved into: Cyano phase SioH PS-DVB Diode Array MS Injection volume: Your comments on your method development approach:Explanation / Answer
Column used must be C18 silica. Size of 4.6*15 cms.
Initial and final gradient = 5-95%
Sample solvent = 50:50 organic:water (methanol or acetonitrile)
Calculate injection volume experimentally.
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