5.00-mL solution aliquots contain PCP were carefully measured into five separate

Question

5.00-mL solution aliquots contain PCP were carefully measured into five separate 50.00-mL volumetric flasks and made basic with KOH. The following volumes of a standard solution of PCP (2.000 µg/mL) were added into each flask: 0.000, 0.500, 1.00, 1.50 and 2.00-mL The fluorimeter readings from each solution are listed below. Calculate the concentration of PCP in the sample.

spike VOl. (mL) spike amount (µg) spike conc.(µg/mL) readout 0.00 0.000 0.0000 3.26 0.500 1.000 0.02000 4.8 1.000 2.000 1.14000 6.41 1.500 3.000 0.06000 8.02 2.000 4.000 0.08000 9.56

Explanation / Answer

Concentration for PCP of 0.5 spike vol. is 0.02

Concentration for PCP of 1.0 spike vol. is 1.14

concentration for PCP of 1.1 spike vol. is 0.06

Concentration for PCP of 2.0 spike vol. is 0.08

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