A Bradford assay can be used to quantify protein in a sample by measuring the ab

Question

A Bradford assay can be used to quantify protein in a sample by measuring the absorbance at 595 nm and comparing it to a standard curve with a known protein concentration. Based on the standard curve below, calculate the concentration (in t ) of a protein sample with an absorbance at 595 nm of 0.418 Standard curve of B SA absorption of light at 595nm to calculate B SA concentration present in sample 0.180 0.180 E 0.140 0120 0.100 0.080 0.000 0.040 0.020 0.000 0.0 20 4.0 6.0 Concentration of BSA (inl) 8.0 10.0 120 Slope = 0.02371 Intercept-0.01132 r2 = 0.973 Note: These values are made up and will differ from those you obtain in lab. Also, remember that your unknown must fall within the range of your standard curve.

Explanation / Answer

from the curve, the equation of best fit is A(absorbance)= concentration*(ug/ml)*0.02371(slope)+ 0.01132 ( intercept)

given A= 0.418, from the equation , 0.418= concentration*0.02371+0.01132

concentration = (0.418-0.01132)/0.02371=17.15 ug/ml

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