A Bradford protein assay was performed on an unknown preparation. A set of BSA s

Question

A Bradford protein assay was performed on an unknown preparation. A set of BSA standard solutions was used to construct a standard curve. The volume of each standard solution assayed was 100ul. The volume of diluted proteinoid and diluted amino acid control assayed was also 100ul each. The absorbances of the standards and diluted unknown are given below: Concentration Absorbance 0 mg BSA 0. 00 5 mg BSA 0. 275 10 mg BSA 0. 560 1:20 unknown sample dilution 0. 260 How much unknown is in one ml of the diluted (1:20) unknown (show all work) and what is the concentration of the undiluted unknown (show all work)? Include slope formula.

Explanation / Answer

25.6 ml

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