Assume the following values of Eo and AGo\': malate + NAD+ oxaloacetate + NADH +

Question

Assume the following values of Eo and AGo': malate + NAD+ oxaloacetate + NADH + H+ lactate + NAD+ pyruvate + NADH + H+ Half reaction Go'z +29.7 k/mol G",= +25.1 kJ/mol Eo (V) Pyruvate + 2H++ 2e. lactate 0.190 Pyruvate + CO2 + H++ 2e malate 0.330 Intracellular steady state concentrations malate-410 M: NADH = 290 M; pyruvate = 3.22 mM: lactate = 1.1 mM; NAD+-20.0 mM; CO2-15.5 torr a) Consider the oxidation of malate to oxaloacetate by NAD: malate + NAD+ oxaloacetate + NADH + H+ In yeast mitochondria, where the pH 8.1, this reaction is exergonic only at low oxaloacetate concentrations. Assuming a pH 8.1, a temperature of 37 °C, and the steady-state concentrations given above, calculate the maximum concentration of oxaloacetate at which the reaction will still be exergonic (b) Now, consider the decarboxylation of oxaloacetate to pyruvate and CO2: oxaloacetate + H+ pyruvate + CO2 Assuming a pH 8.1, a temperature of 37 C, and the steady-state concentrations given above, is this decarboxylation reaction thermodynamically favorable or not at the oxaloacetate concentration you calculated in part (a)? Show the calculations that support your answer.

Explanation / Answer

Assume the following values of Eo and AGo': malate + NAD+ oxaloacetate + NADH +

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