Sprint LIE 6:57 PM gastate.view.usg.edu Lisa is running an experiment to determi

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Sprint LIE 6:57 PM gastate.view.usg.edu Lisa is running an experiment to determine the kinetic parameters (Km and Vx) of the enzyme that she is studying, She has run a series of experiments where she changed the amount of substrate added and measured the accumulation of product over a 5 second time interval. She used this data to calculate the dependence of the rate of the reaction on substrate concentration. Her results listed below Substrate Concentration (mM)Velocity (mM/sec) 2.5 2 5 10 20 6.3 7.6 1. Using this information construct a Michaelis-Menten curve 2. Using this information construct a Lineweaver-Burk plot. 3. Using the Lineweaver-Burk plot determine the values of K. and Vm To learn more about how this enzyme functions Lisa decides to perform an experiment using two different types of inhibitors. She has collected the data below. Substrate Concentration Velocity Va mM 2.5 1.17 2.1 0.77 .25 6.3 7.6 20 7.2 2.86 1. Using this information construct a Michaelis-Menten curve that contains the data for all three data sets (velocity, va and va).All three data sets should be on the same graph. 2. Using this information construct a Lineweaver-Burk plot for all three data sets (velocity, va and va). All three data sets should be on the same graph 3. Using the Lineweaver-Burk plot determine the values of K and Vmax for the new data sets for all three data sets (Va and va). 4. What type of inhibitor (competitive, noncompetitive or neither) is Inhibitor (S,Vi)and Inhibitor(Sa). Explain your answer.

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Sprint LIE 6:57 PM gastate.view.usg.edu Lisa is running an experiment to determi

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