3. You discover two of the three zebrafish genes for yellow pigment production a

Question

3. You discover two of the three zebrafish genes for yellow pigment production are expressed in your frog. You decide to look for mutations in these genes by amplifying each gene by PCR and sequencing the PCR products by Sanger sequencing to determine if any of these genes are mutant in your blue frog

a. Review the three steps of PCR and describe what happens at each step.

b. Which step in PCR is analogous to the initiation step of DNA replication?

c. Draw the molecule that enables chain termination in Sanger sequencing. What is different about this molecule and the structure with the molecule typically used in DNA synthesis? How does this difference result in terminating DNA synthesis?

Explanation / Answer

b. Annealing, because that is when the polymerases attach c. Here's the picture http://pds7.egloos.com/pds/200710/14/29/e0058929_4711e194e610b.jpg The analog The analog can't be extended

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