Can anyone elxplain to me how in the figure below the loss of ion channel activi

Question

Can anyone elxplain to me how in the figure below the loss of ion channel activity (TRPV3) is lost?

Caption that came with figure:

TRPV3 agonist cocktail (200 mM2-APB + 500 mMCarvacrol) induced large increases of [Ca2+]i in primary cultured keratinocytes isolated fromWT(V3+/+) but not V3KO(V3/) mice. Whereas more than 80%of WT keratinocytes responded strongly to the V3 agonist cocktail, negligible responses were observed for V3KO keratinocytes. Positive controls:60%–80% keratinocytes from both genotypes (WT and V3 KO) responded to 4a-PDD (3 mM; agonist of TRPV4). All cells responded to ionomycin (1 mM).

Explanation / Answer

Application of  TRPV3 agoinst cocktail (200 M 2-APB + 500 M Carvarcol) induced large increase of Ca2+ ion in primary cultured keratinocytes isolated from wild type (WT or V3+/+) but not V3 KO (V3-/-) because the removal of extrernal Ca2+ abolish most of the agonist, which effects the Ca2+ responses. Hence, there is no singnificant increase in Ca2+ in primary keratinocytes V3 KO. Keratinocyte TRPV3 sensitivity depends on the Ca2+ -permeant channels, which forms a signaling complex with TGF-/EGFR.

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