Name Section LAB Effects of Environmental Variables on the Rate of Photosynthesi

Question

Name Section LAB Effects of Environmental Variables on the Rate of Photosynthesis Problem sthe effect of varying light intensity or temperature on the rate of photosynthesis? Objectives After completing this exercise the student will be able to: 1. Dis 2. List other 3. Write an equation for the overall reaction of photosynthesis 4. cuss the relationship between light intensity and the rate of photosynthesis. parameters that may influence the results of the rate of photosynthesis. Conduct an additional investigation of another environmental variable and its effect on the rate of photosynthesis. Preliminary Information Green plants possess the unique ability to trap solar energy and use it to convert CO, and H,0 into sunlight chlorophyll Since O, is a byproduct of photosynthesis, the rate of O2 given off can be used as a means of determining Using the volumeter, the relative amounts of O, given off by an aquatic plant under different conditions carbohydrates and O2·This is photosynthesis and this overall reaction is usually written: the rate of photosynthesis. can be measured. During photosynthesis carbon dioxide (CO) is taken in by the plant. To use a volumeter for detecting the oxygen given off by the plant, it is necessary to provide the plant with an adequate amount of CO2. This is done by placing the plant in a 1% sodium bicarbonate (NaHCO) solution. The NaHCO, will go through a chemical change and replace the CO, that the plant takes from the water. The O, that the plant gives off as it carries on photosynthesis will be released into the air above the water. This will cause a volume change that will register on the volumeter he temperature will be monitored in case the temperature increases to a point that enzymes in the plant are denatured, causing an immediate drop in the rate of photosynthesis. 63

Explanation / Answer

   Effect of varying light intensity/temperature on rate of photosynthesis

Introduction:

                 Green plants posses the unique ability to trap solar energy and use it to convert CO2 and H2O into carbohydrates and O2. This is photosynthesis. Oxygen is the by product of photosynthesis, the rate of oxygen can be given off can be used as a means of determining the rate of photosynthesis. During photosynthesis carbon dioxide is taken in by the plant.

Data:

              The data for the effect of light intensity are 10cm, 14cm, 20cm, and 32cm. The net oxygen production is -3mm, -1mm, -1mm.

Hypothesis:

I believe that light intensity will increase the photosynthetic rate

I believe that temperature will decrease the rate of photosynthesis

Process:

Place an Elodea plant upside down in the sodium bicarbonate solution. Prepare a control by placing an equal amount of sodium carbonate solution without a plant in other test tube. The size of air space in this test tube should be identical to that in the test tube with the plant. Put the Elodea and control in the jar. The movement of a drop of dye along a glass tube will be used to measure oxygen production. Place a drop of 1-2 cm long drop of dye in each of the long glass tubes. Attach the rubber tubing from the Elodea and control tubes to each of the long glass tubes. Using masking tape, tape the long glass tubes to the table. Place a flood lamp 32cm from the plant. Place a flood lamp 32cm from the plant. Using a small piece of masking tape, mark the starting point of the dye. Turn the lamp on and begin the experiment. Take measurements every 2 min for a 10 min period. Do not move the masking tape during the 10 min run. Reposition the lamp 20, 14, 10cm from the plant and return the experiment.

Conclusion

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