DATA SHEET 6-1 Name Date Lab Section I was present and performed this exercise (
ID: 132207 • Letter: D
Question
DATA SHEET 6-1 Name Date Lab Section I was present and performed this exercise (nitials) (continued) 11 Plating 1.0 ml of a sample diluated by Plating 1.0 ml of a sample diluted by a factor of 10-1 produced 43 colonies, What was the original cell density in the sample? 12 P Plating 0.1 ml of a sample diluted by a factor of 1O 1 produced 43 colomies What was the original cell density in the sample? 13 A plate with a sample volume of 10- ml produced 72 colonies a. What was the original cell density b. How many colonies should be on the plate imoculated with a sample volume of 10"s mL c. How mamy colonies should be on the plate imoculated with a sample volume of 10 mL 14 A plate with a sample solume of 10 *ml,produced 259 colomies a. What was the original cell density? b. How mary colonies should be on the plate inoculated with a sample volume of 10- mL c. How many colonies should be on the plate inoculated with a sample volume of 10-7ml A mutrient agar plate that received 1,000 ul of a bacterial sample diluted by a factor of 10& had 298 colonies on it after incubation. What was the original cell density?Explanation / Answer
11) One mL of the solution diluted by a factor of 10^-3 produced 43 colonies.
The original cell density in the sample is calculated by the formula,
= number of colonies /dilution factor
= 43/10^-3
= 43 x 10^3
= 43000 CFU/mL
The cell density present in the original sample is 43000 CFU/mL
12) 0.1 ml of sample diluted by a factor of 10^-3 produced 43 colonies.
Therefore, the original cell density is,
= 43/10^-3 per 0.1 ml
= 43 x 10^3 per 0.1 ml
= 43 × 10^4 CFU/mL
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