8. What is true regarding SNP (single nucleotide polymorphism) and SSR (simple s

Question

8. What is true regarding SNP (single nucleotide polymorphism) and SSR (simple sequence repeat)? A. Most of them do not influence phenotypes B. SNP is more commonly found than SSR in the genome. They can both be detected through sequencing of the PCR product that contains the polymorphism locus. D. An individual can have two same alleles or two different alleles for a polymorphism locus E. All of the above 9-10. Shown below is part of the last protein-coding exon of the CFTR gene. 5 GGCTAAGATCTGAATTTTCCGAG... .TTGGGCAATAATGTAGCGCCTT 3 9. Which of the following sets of primers could you use to amplify the entire target DNA sequence shown above? A. 5' GGCTAAGATC 3 and 3' CCGATTCTAG 5 B. 5' GTAGCGCCTT 3 and 3' CCGATTCTAG 5 C. 5' GGCTAAGATC 3 and 3' CATCGCGGAA 5 D. 5' GTAGCGCCTT 3' and 3 CATCGCGGAA 5

Explanation / Answer

Ans:

Q8: Answer seems to be ‘e’all of the above.

Beacasue: Microsatellite or short tandem repeats (STRs) or simple sequence repeats (SSRs) exist in thousands.

Explanation:

a. most of the SNPs are in non-coding regions (in human geneome > 85%) hence SNPs do not influence phenotypes hence this choice also true. b. Most of the SNPs are found in SSRs only, and SSRs exist in thousands throughout the genome and most of SSRs are in non-coding repetitive regions hence this choice also true. C. This choice also true can be detected by PCR. d. this choice also true

Q9-10 your selection ‘C’ is the right answer 5’-3’ and 3’ – 5’ primers are complimentary to template DNA

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