Two pure breeding plants with the genotypes ddPP and DDpp are crossed together t

Question

Two pure breeding plants with the genotypes ddPP and DDpp are crossed together to generate F1 progeny with the genotype DdPp. F1 individuals were testcrossed to a homozygous parental strain (ddpp). The following data were obtained: Genotype observed # of progeny Expected # of progeny DdPp 190 Ddpp 210 Ddpp 310 ddPp 290 Total: 1000

A. In the table above, fill in the values for the expected number of progeny for each genotype based on the hypothesis that D and P are unlinked.

B. What is the calculated ? 2 value?

C. How many degrees of freedom are there?

D. Are D and P linked or unlinked? (Use the ? 2 table attached below).

E. Briefly explain how the ? 2 analysis supports your conclusion.

Explanation / Answer

1.

Calculated Chi square value = 41.6

Degree of freedom = n-1 = 3

Chi square table value is 7.81.

Both D and P are unlinked.

Calculated Chi square value > Chi square table value.

So, reject null hypothesis.

Observed Expected O-E (O-E)2 (O-E)2/E DdPp 190 1000 X 1/4 = 250 -60 3600 14.4 Ddpp 210 250 -40 1600 6.4 Ddpp 310 250 60 3600 14.4 ddPp 290 250 40 1600 6.4 Total 1000 41.6

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