Two proteins have been purified by a researcher, called Bucky A and Bucky B. She

Question

Two proteins have been purified by a researcher, called Bucky A and Bucky B. She determines the extinction coefficient for each, generating the following information:

5. (3 points) Two proteins have been purified by a researcher, called Bucky A and Bucky B. She determines the extinction coefficient for each generating the following information Bucky A: 1 mg/ml solution OD280 1.1 Bucky B 1 mg/ml solution OD280 0.27 She then purifies a new preparation of Bucky A. Her new preparation of Bucky A has 4 ml total, and when it is diluted 1/10 it gives OD280 0.44. The total protein in her new preparation of Bucky A mg Insert the words "higher" or lower Bucky B has a fraction of Trp residues than the average protein

Explanation / Answer

The standard curve equation: y = 0.012x

where y is the absorbance and X is the protein

so x = y/0.012

= 0.44/0.012

=36.6

but it is diluted 1/10, so the total protien is 366 ug/ ml

Bucky B has a lower fraction of Trp residues that the average protein. Because, the aminoacids of aromatic rings tryptophan absorb at higher wavelength.

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