Name: Date: ELISA ASSESSMENT The following picture represents test results from
ID: 133399 • Letter: N
Question
Name: Date: ELISA ASSESSMENT The following picture represents test results from blood samples of two patients A and B. Patient A has been vaccinated against influenza virus 12 weeks ago, while B has received the same vaccine 48 hours ago. Both samples were tested for presence of antibodies to influenza HA antigen using ELISA. (HA is a protein on the surface of the influenza virus, that helps with attachment.) The same protocol was followed as shown in the video under the protocol section for ELISA 1. Positive control (HA present) Negative control (HA absent) Sample A Sample B What does the negative control indicate? Or what information can you gather from the negative control? E. F. As performed, is this a qualitative test or a quantitative test? The results show that each assay was performed 3 times (in triplicate). Why do you think this assay was performed in triplicate? G. H. At this point and time, if A and B would be exposed to influenza virus, who would be better protected?Explanation / Answer
E. The negative control make sure that there is no cross reaction between the reagents used in experiment that can cause the result to be false positive, the negative control is serum sample without any influenza virus or the antibody which you wish to detect.
F. It is qualitative Elisa since we can observe only result in form of positive or negative, in quantitative Elisa serial dilution of sample or serum is done which has antibodies.
G .Always an experiment for Elisa is done in triplicate just to make sure that if result in all 3 test are same then it’s done properly otherwise their might be some laboratory or human error in technique.
H. If both sample are exposed to influenza virus sample A would be better protected since antibodies against influenza develop after 2 weeks and the person has taken vaccine 12 weeks ago so antibodies must have developed which will give protection while in second case its only 48 hours so antibody might have not developed that can give protection.
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