13) Replica plating is an example of A) positive selection B) negative selection

Question

13) Replica plating is an example of A) positive selection B) negative selection C) screening D) counterselection E) none of the above. 14) We now know that bacterial cells inside a single colony are not genetically identical, though they could in theory come from a single cell. This is mainly due to A) spontaneous mutation B) induced mutation C) transcription D) radiation E) UV light 15) Frederick Griffith infected mice with a combination of dead (encapsulated pathogenic) R and live (non-encapsulated) S bacterial strains. What was the outcome, and why did it occur? A) The mice will live. Transformation was not required B) The mice will die. Transformation of genetic material from R to S was required. C) The mice will live. Transformation of genetic material from S to R was required. D) The mice will die. Transformation was not required E) None of the above is correct. 16) Competence is the ability of A) recipient cells to take up DNA fragments. B) donor cells to transfer DNA fragments. C) plasmids to be spliced with a foreign DNA. D) F+and F- cells to conjugate E) phage to carry chromosomal DNA fragments. 17) Which of the following is NOT a method of horizontal gene transfer? A) integration of a transposon B) chromosomal transfer via conjugation C) transduction D) transformation E) plasmid transfer via conjugation

Explanation / Answer

13. replica plating is an example of _____

B) negative selection only

Negative (indirect) selection detects mutant cells because they do not grow

14.

A) spontaneous mutation

15. B

16. A

the ability of a recipient cell to take up Extracellular DNA from the environment; bacteria need this in order to transform

17. E

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