answers this questions The PCR Product in Your Tube In Stage 2 you used PCR to a

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The PCR Product in Your Tube In Stage 2 you used PCR to amplify a segment of the hTAS2R38 (bitter taste) gene. In your PCR tube you now have more than a billion copies of the segment between the start of the forward primer and the start of the reverse primer (see Figure 1) Figure 1 The forward primer, highlighted in orange, starts at the* CGGGGATGAC TAAGACACCG CGCTGITITA TCCCCACTAC CAAACACAAC CCTATTACCG TCGAACAGGG AGACCCGTAC GTCGGCAGGA CTAGAGTCCG TTACGGTTCA ACTCCTCTCG ACACTACTGG TAAGACGAGA CCCGAGTCTC 961 GTAAGAGCCG ACCACAAGGC AGATTCCCGG ACACTOTGCT GA 3 CATTCTCGGC TCTAAGGGCC TGTGACACGA CT The reverse primer, highlighted in orange, starts at the 1. At which base pair (bp) position does the forward primer start (*)? position At which bp position does the reverse primer start ()? position How could you figure out how long the PCR product is? How many bps long is the PCR product?

Explanation / Answer

Please find the answers below:

Answer 1:

Part 1: Position 838 (since the primer location and complementarity at this position would be maximum, position 838 serves as the best location for binding of forward primer)

Part 2: Position 878 (since the primer complementarity refers to sequence 878, it serves as the binding location for reverse primer)

Part 3: The size of the PCR-product can be found be simply resolving a molecular weight marker along with the PCR amplicon. The relative location of the PCR band across the gel with respect to the molecular weight marker refers to the size of PCR product.

Part 4: 140 base pairs (According to the image, each group of nucleotides contains 10 bases, and hence total of 140 bases in 14 groups)

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