Genetic Engineering Challenge 2. Locate the SNP at position 886 in the segment o

Question

Genetic Engineering Challenge 2. Locate the SNP at position 886 in the segment of the hTAS2R38 (bitter ta ste gene in Figure 2. Circle the sequence of the bases from 883 to 8B Figure 2 836 881 CAGC 37 Aatil 5...-G A C G Tk 3 CT GCAG... 5' 3 C T T A A G . 5, 3. TTCGA A... 5' EcoRI Sticky Ends 3. Compare the recognition sequence for Aatll in Figure 3 to the sequence between bases 883 and 888. What do you notice? 4. Why do you think we have chosen Aatll for this job? 5. What do you think is the problem here?

Explanation / Answer

Ans-2 the bases between 883-888 are as follows

GCC GTC

Ans-3 here the SNP is same as it was in figure 2 but the bases prior to SNP have changed. It have GAC at the place of GCC.

Ans-4 Aatil is choose because it also has the same SNP as given in figure 2 so it can be used for comparison or identification of some mutation .

Ans-5 here may be some problem related to location of some specific gene or SNP in the genome.

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